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human inflammatory cytokines multianalyte elisarray kit  (Qiagen)
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Qiagen human inflammatory cytokines multianalyte elisarray kit
Beta-Defensin 124 (DEFB124) upregulation induces increased production of <t>cytokines</t> and chemokines. DEFB124 promotes mRNA expression for cytokines (A) and chemokines (B) in DEFB124-induced RWPE-1 cells. The mRNA expression of these genes was determined by using quantitative real-time polymerase chain reaction. Relative expression levels of each gene were calculated from cycle threshold values and were normalized with ACTB, and the expression ratio was calculated against the expression of each gene in the empty vector-transfected RWPE-1 cells. Experiments were repeated at least three times, and data are expressed as the mean±standard error of the mean (SEM). Asterisks, * and ** , represent statistical significance at p<0.05 and p<0.01, respectively. (C) DEFB124 is required for cytokine and chemokine production. Supernatants from empty vector- or DEFB124-DDK-Myctransfected RWPE-1 cells were collected. The concentrations of cytokines and chemokines in supernatant were measured by <t>multianalyte</t> enzyme-linked immunosorbent assay (ELISA). The diagram shows mean ELISA absorbance values (450 nm) for triplicates with the error bars representing the SEM. Asterisks ( *, ** , and *** ) represent statistical significance at p<0.02, p<0.005, and p<0.0001, respectively. IL, interleukin.
Human Inflammatory Cytokines Multianalyte Elisarray Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beta-Defensin 124 (DEFB124) upregulation induces increased production of cytokines and chemokines. DEFB124 promotes mRNA expression for cytokines (A) and chemokines (B) in DEFB124-induced RWPE-1 cells. The mRNA expression of these genes was determined by using quantitative real-time polymerase chain reaction. Relative expression levels of each gene were calculated from cycle threshold values and were normalized with ACTB, and the expression ratio was calculated against the expression of each gene in the empty vector-transfected RWPE-1 cells. Experiments were repeated at least three times, and data are expressed as the mean±standard error of the mean (SEM). Asterisks, * and ** , represent statistical significance at p<0.05 and p<0.01, respectively. (C) DEFB124 is required for cytokine and chemokine production. Supernatants from empty vector- or DEFB124-DDK-Myctransfected RWPE-1 cells were collected. The concentrations of cytokines and chemokines in supernatant were measured by multianalyte enzyme-linked immunosorbent assay (ELISA). The diagram shows mean ELISA absorbance values (450 nm) for triplicates with the error bars representing the SEM. Asterisks ( *, ** , and *** ) represent statistical significance at p<0.02, p<0.005, and p<0.0001, respectively. IL, interleukin.

Journal: Korean Journal of Urology

Article Title: Beta-Defensin 124 Is Required for Efficient Innate Immune Responses in Prostate Epithelial RWPE-1 Cells

doi: 10.4111/kju.2014.55.6.417

Figure Lengend Snippet: Beta-Defensin 124 (DEFB124) upregulation induces increased production of cytokines and chemokines. DEFB124 promotes mRNA expression for cytokines (A) and chemokines (B) in DEFB124-induced RWPE-1 cells. The mRNA expression of these genes was determined by using quantitative real-time polymerase chain reaction. Relative expression levels of each gene were calculated from cycle threshold values and were normalized with ACTB, and the expression ratio was calculated against the expression of each gene in the empty vector-transfected RWPE-1 cells. Experiments were repeated at least three times, and data are expressed as the mean±standard error of the mean (SEM). Asterisks, * and ** , represent statistical significance at p<0.05 and p<0.01, respectively. (C) DEFB124 is required for cytokine and chemokine production. Supernatants from empty vector- or DEFB124-DDK-Myctransfected RWPE-1 cells were collected. The concentrations of cytokines and chemokines in supernatant were measured by multianalyte enzyme-linked immunosorbent assay (ELISA). The diagram shows mean ELISA absorbance values (450 nm) for triplicates with the error bars representing the SEM. Asterisks ( *, ** , and *** ) represent statistical significance at p<0.02, p<0.005, and p<0.0001, respectively. IL, interleukin.

Article Snippet: Cytokines and chemokines were measured by using the human inflammatory cytokines multianalyte ELISArray kit (Qiagen) and human common chemokines multianalyte ELISArray kit (Qiagen) according to the manufacturers' instructions, respectively.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Plasmid Preparation, Transfection, Enzyme-linked Immunosorbent Assay

Beta-Defensin 124 (DEFB124)-mediated upregulation of cytokines and chemokines promote chemotactic response of THP-1 monocytes. DEFB124- or DEFB124-mediated cytokines and chemokines induce chemotaxis for THP-1 monocytes. THP-1 cells (1×10 6 cells) were added to the upper chamber, and the lower chamber contained supernatants from either the empty vector- or DEFB124-DDK-Myc-transfected RWPE-1 cells. The results are presented as a migration index denoting the fold increase of cell migration over the empty vector. Monocyte chemoattractant protein-1 (MCP-1) (100 ng/mL) was used as a positive control. Results are representative of three independent experiments. Asterisk represents statistical significance at p<0.02.

Journal: Korean Journal of Urology

Article Title: Beta-Defensin 124 Is Required for Efficient Innate Immune Responses in Prostate Epithelial RWPE-1 Cells

doi: 10.4111/kju.2014.55.6.417

Figure Lengend Snippet: Beta-Defensin 124 (DEFB124)-mediated upregulation of cytokines and chemokines promote chemotactic response of THP-1 monocytes. DEFB124- or DEFB124-mediated cytokines and chemokines induce chemotaxis for THP-1 monocytes. THP-1 cells (1×10 6 cells) were added to the upper chamber, and the lower chamber contained supernatants from either the empty vector- or DEFB124-DDK-Myc-transfected RWPE-1 cells. The results are presented as a migration index denoting the fold increase of cell migration over the empty vector. Monocyte chemoattractant protein-1 (MCP-1) (100 ng/mL) was used as a positive control. Results are representative of three independent experiments. Asterisk represents statistical significance at p<0.02.

Article Snippet: Cytokines and chemokines were measured by using the human inflammatory cytokines multianalyte ELISArray kit (Qiagen) and human common chemokines multianalyte ELISArray kit (Qiagen) according to the manufacturers' instructions, respectively.

Techniques: Chemotaxis Assay, Plasmid Preparation, Transfection, Migration, Positive Control